Pulsed electron-nuclear double resonance (ENDOR) at 140 GHz.

نویسندگان

  • M Bennati
  • C T Farrar
  • J A Bryant
  • S J Inati
  • V Weis
  • G J Gerfen
  • P Riggs-Gelasco
  • J Stubbe
  • R G Griffin
چکیده

We describe a spectrometer for pulsed ENDOR at 140 GHz, which is based on microwave IMPATT diode amplifiers and a probe consisting of a TE011 cavity with a high-quality resonance circuit for variable radiofrequency irradiation. For pulsed EPR we obtain an absolute sensitivity of 3x10(9) spins/Gauss at 20 K. The performance of the spectrometer is demonstrated with pulsed ENDOR spectra of a standard bis-diphenylene-phenyl-allyl (BDPA) doped into polystyrene and of the tyrosyl radical from E. coli ribonucleotide reductase (RNR). The EPR spectrum of the RNR tyrosyl radical displays substantial g-anisotropy at 5 T and is used to demonstrate orientation-selective Davies-ENDOR.

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عنوان ژورنال:
  • Journal of magnetic resonance

دوره 138 2  شماره 

صفحات  -

تاریخ انتشار 1999